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KMID : 0364820130490020131
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Korean Journal of Microbiology
2013 Volume.49 No. 2 p.131 ~ p.136
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Screening of Rhizobium, Hairy Vetch Root Nodule Bacteria, with Promotion of Nodulation and Nitrogen Fixation
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Jang Jong-Ok
Kwon Mi-Kyung
Park Dong-Jin
Sung Chang-Keun
Kim Chang-Jin
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Abstract
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This study was conducted to select rhizobia from hairy vetch (Vicia villosa Roth) with nodulation and excellent nitrogen-fixing ability. Hairy vetch root was collected from 7 of cultivation region of all over the country, rhizobia were isolated from the root nodules. Isolates were re-inoculated into a hairy vetch separately and studied nodulation and nitrogen-fixing ability. As a result, total of 52 Rhizobium isolates were isolated from the hairy vetch root nodules, among these, 16 isolates were Rhizobium which show good growth at more than 0.5% NaCl concentration.
These 16 isolates were re-inoculated separately, 8 weeks after, good root nodule formation was observed from Rhizobium sp. RH1, RH3, RH81, RH82, RH84, and RH93 strain treated samples. Six isolates were positive for nitrogen fixing ability, the highest acetylene reduction activity was shown by Rhizobium sp. RH84. Results suggest that the Rhizobium sp. RH84 could be used as the possibility of its application as a green manure crop of hairy vetches in nonuniform salt distribution reclaimed land.
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KEYWORD
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Rhizobium sp., hairy vetch, nitrogen fixation, root nodule
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